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KMID : 0604520190450010057
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Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 1 p.57 ~ p.67
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Role of Dual Oxidase 2 in Reactive Oxygen Species Production Induced by Airborne Particulate Matter PM10 in Human Epidermal Keratinocytes
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Seok Jin-Kyung
Choi Min-A
Ha Jae-Won
Boo Yong-Chool
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Abstract
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Particulate matters with a diameter of < 10 μm (PM10) exert oxidative stress and inflammatory events in various organs. The purpose of this study was to examine the molecular mechanism of reactive oxygen species (ROS) production induced by PM10 in the human epidermal keratinocytes (HEKs). When cultured HEKs were exposed to PM10, ROS production was induced and it was inhibited by apocynin, an antioxidant. The mRNA expression of NADPH oxidase (NOX) family was analyzed in order to examine their role in PM10-induced ROS production. PM10 increased the mRNA expression of NOX1, NOX2, dual oxidase (DUOX) 1 and DUOX2. HEKs expressed DUOX1 and DUOX2 at higher levels compared to other NOXs. The mRNA expression of dual oxidase maturation factors, DUOXA1 and DUOXA2, was also increased by PM10. We examined whether these calcium-dependent enzymes, DUOX1 and DUOX2, mediate the PM10-induced ROS production. A selective intracellular calcium chelator, BAPTA-AM, attenuated ROS production induced by PM10 or calcium ionophore A23187. The small intereference RNA (siRNA)-mediated down-regulation of DUOX2, but not DUOX1, attenuated the ROS production induced by PM10. PM10 increased the expression of inflammatory cytokines such as interleukin (IL)-1β, IL-6, IL-8 and interferon (IFN)-γ. SiRNA-mediated down-regulation of DUOX2 suppressed the PM10-induced expression of IFN-γ but not other cytokines. This study suggests that DUOX2 plays a crucial role in ROS production and inflammatory response in PM10-exposed keratinocytes.
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KEYWORD
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airborne particulate matters, dual oxidase 2, reactive oxygen species, calcium ion, keratinocytes
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